Advisory Board

Samuel Asamoah Sakyi
Email : ssakyi@noguchi.ug.edu.gh
Phone : (+233)244530214
Fax :
Department : Bacteriology
 
 
Biography

Samuel Asamoah Sakyi has a Bachelors of Science Degree in Medical Laboratory Technology and a PhD in Immunology of infectious disease from the Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana. He also has a graduate certificate in Fundamentals of Molecular Pathology from the American Association of Clinical Chemistry (AACC). Dr Sakyi is a recipient of many local and international awards and has a wealth of experience in clinical laboratory assays with specialty in clinical chemistry.

 

Project Name

Selection and application of aptamers as a simple diagnostic tool for buruli ulcer

 
Research Interest

My research interest focuses on identifying biomarkers for early and accurate diagnosis of infectious and chronic diseases. Early diagnosis of disease is important for effective treatment to prevent the morbid effects of the disease. My PhD work investigated protein and genetic predisposition biomarkers among Ghanaians presenting with Rheumatoid Conditions. In partnership with Axis Shield, UK, native citrulline-containing peptides were modified into citrullinated peptide using peptidylarginine deiminases (PADIs). The resulting anti-cyclic citrullinated peptide (anti –CCP) was then compared with conventional Rheumatoid factor latex and its isotypes RF-IgA and RF-IgM. The study revealed that anti –CCP is a better marker. Moreover, HLA-B27 and its subtypes were found to be present among Ghanaians presenting with Ankylosing  Spondylitis and serves as genetic predisposition factor, but differs in clinical and biochemical presentation.
I am currently working on how to select aptamers against mycolactone or any of the Mycobacterium ulcerans elicit proteins using the SELEX process to develop a point of care diagnosis tool for early detection of buruli ulcer, a neglected tropical disease.
Future work will include how to determine and standardized vitamin D assays, first among the general population then among specific groups of patients using LC/MS-Mass Spectrometry.

 
Projects

Contribution of host and parasite diversity to malaria transmission in Ghana.

 
Publications

Samuel A. Sakyi, Margaret Agyei Frimpong and Robert E. Quansah (2012)HLA-B27 and its Associated Clinical and Biochemical Presentation among Ghanaians with Ankylosing Spondylitis (J. Med Sci., 12 (6) 161-167)

Margaret Agyei Frimpong, Samuel A. Sakyi and Robert E. Quansah (2010)
Comparison of anti-CCP with RF and its isotypes for early and differential diagnosis of Rheumatoid Arthritis. J. Med Sci., Vol 10 issue 1 page no. 19-24

Samuel A. Sakyi, Richard K.D Ephraim, Enoch O. Antoh, Christian Obirikorang and Gifty O. Berchie (2012) Lipid Peroxidation and Catalase Levels among Children Presenting with Severe  falciparum Malaria in the Sefwi Wiawso Municipality, Ghana (J. Med Sci, Vol 12 (5) 141-147)

 
Dr. Kwadwo Asamoah Kusi
DR. Evelyn Yayra Afua Bonney Kingsley Badu
     
Dr Aminata Colle LO Samuel Asamoah Sakyi Dr. Jewelna Akorli
(nee Osei-Poku)
     
Dr Patrick Valere Tsouh Fokou
Dr. Patrick Kobina Arthur Dr. Joseph Mwanzia Nguta
     
 
Dr Edward Maina Protus Arrey Tarkang